Journal: Stem Cells International
Article Title: The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level
doi: 10.1155/2022/9346767
Figure Lengend Snippet: MHC class-I expression level. (a) Histograms shows the real-time PCR analysis of Qa-1 , Qa-2 , H2kb , H2kd , H2db , and H2dd in TSA-treated ESCs and iPSCs vs. nontreated control groups and parental MEF cells. (b) Flow cytometry analysis of the expression of MHC I molecules on the surface of ESCs, iPSCs, and MEF cell. ns: not significant; ∗∗ and ∗∗∗ indicate the statistical significance, P < 0.01, P < 0.001, respectively, compared to the control group.
Article Snippet: Major histocompatibility complexes were detected using antibodies against MHC class I molecules (MHCI mAb unconjugated, cat # NB120-6405, Novus) and MHC class II molecules (MHCII mAb unconjugated cat # sc-32247, Santa Cruz).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry